Human Normal Rectum Lysate

Name Human Normal Rectum Lysate
Supplier ABBIOTEC
Catalog 450197
Category Tissue Lysate
Prices $105.00
Sizes 100 µg
Applications WB
Species Human
Tissue Rectum
Disease Normal
Description Rectum tissue lysate was prepared by homogenization in homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
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